Butterfly dispersal in inhospitable matrix: rare,risky, but long-distance

Metapopulation models typically assume that suitable habitats occupied by local populations and unsuitable matrix separating them form a ‘black-and-white’ landscape mosaic, in which dispersal is primarily determined by the spatial configuration of habitat patches. In reality, however, the matrix composition is also likely to influence dispersal. Using intensive mark-recapture surveys we investigated inter-patch movements in Maculinea (Phengaris) nausithous and M. teleius occurring sympatrically in six metapopulations. Three of these metapopulations had the matrix dominated by forest, an inhospitable environment for grassland butterflies, whereas in the remaining three the matrix was mostly composed of open environments. Dispersal parameters derived with the Virtual Migration model revealed significant differences between both groups of metapopulations. Both species had a lower propensity to emigrate from their natal habitat patches, and they suffered substantially higher dispersal mortality in the metapopulations with forest matrix. On the other hand, mean dispersal distances were roughly an order of magnitude longer in forest matrix as compared with open landscapes (ca. 500–1,500 vs. 100–200 m). Our results suggest that inhospitable forest matrix induces strong selection against dispersal, leading to a reduced emigration rate. At the same time, the selection may promote emigrants with good dispersal abilities, which are able to perform long-distance movements. Thus, while it is generally believed that a matrix structurally similar to the habitat of a species should improve the functional connectivity of habitat patches, our findings imply that this may not necessarily be the case.     

Distribution of polycyclic aromatic hydrocarbons (PAHs) in the bottom sediments of a dam reservoir,their interaction with organic matter and risk to benthic fauna

Journal of Soils and Sediments - The aims of the study were to investigate the interaction between fractions of organic matter and polycyclic aromatic hydrocarbons (PAHs) in bottom sediments and to...     

15Nitrogen Study on Accumulation and Allocation of Nitrogen Applied at Anthesis on Top of Early Nitrogen Applications in Winter Wheat

The objective of this experiment was to assess the effect of interaction between early and late nitrogen (N) fertilization on grain yield, protein content, and distribution of N between organs of winter wheat. Wheat was grown in pots in vegetation house. Experiment has been carried on in three years 2001–2003, each year in four replications. Wheat supplied with 0.9, 1.4, 1.9, 2.4, and 2.9 g N/pot was top dressed at anthesis by 0.85 g labeled nitrogen ¹⁵N/pot. Plants supplied with 0.9 g N/pot enhanced grain yield by 28% and gluten content by 62% and plant supplied with 2.9 g N/pot by 27% and 13%, respectively. The amount of ¹⁵N fertilizer stored in plant organs decreased proportionally to the amount of previously accumulated nitrogen, but the share of ¹⁵N in plant parts was almost constant. Grain accumulated about 86% of total ¹⁵N taken up by plants. Irrespective on nitrogen status prior to anthesis, about 50% of ¹⁵N has been used for gluten formation. The percentage of ¹⁵N recovery fraction ranged from 79% to 86%.     

Community structure of Lumbricidae in beech woodland of the Bieszczady National Park, Southeast Poland

As ecosystem engineers, earthworms play a key role in the soil environment. However, due to increasing anthropogenic pressure, soil organisms,including earthworms, are being threatened by habitat loss. In this study, we undertook a qualitative and quantitative investigation of earthworms of the family Lumbricidae in four types of Carpathian beech woodland(Fagetum carpaticum), characterized by their understory vegetation(I, F. c. festucetosum drymejae; II, F. c. typicum; III, F. c. lunarietosum; and IV, F. c. allietosum), in the Bieszczady National Park(Eastern Carpathians, Southeast Poland). At each investigated site, soil monoliths(25 cm × 25 cm × 25 cm) were examined by hand sorting. Earthworms were expelled from deep soil layers using a weak formalin solution(0.4%). Depending on the phytocoenosis, 7 species of Lumbricidae were identified at each of the sites I, II, and III and 10 at site IV. Site IV(F. c. allietosum) differed significantly(P 0.05) from the other three sites with respect to earthworm biomass(59.71 ± 39.53 g m~(-2))and the Shannon-Wiener diversity index(0.52 ± 0.12). Although present three decades ago, the deep-burrowing species Octodrilus transpadanus and the litter-dwelling species Dendrobaena octaedra were not found at site IV in the present study. We suspect that these two species may have been displaced by the invasive Lumbricus terrestris, which was not found at the same site in the 1980 s. Such observations warrant further investigation to verify the predicted effects of an invasive earthworm, including the potential effects on soils and other fauna and flora, which have been documented in numerous countries.     

The effect of feeding on oxygen consumption and ammonia excretion of juvenile tench Tinca tinca (L.) reared in a water recirculating system

The effect of feeding frequency (one, three, and continuous feeding), feed ration (0.2, 0.5, 0.8% of total fish biomass), and feeding per se on the oxygen consumption (OC, mg O₂ kg⁻¹ h⁻¹) and ammonia excretion (AE, mg TAN kg⁻¹ h⁻¹) of juvenile tench (body weight 15–19 g) and variations in these parameters in daily cycles were examined. Fish metabolism was studied in a recirculating system (rearing tanks of 0.2 m³, water temperature 23 °C). It was found that oxygen consumption and ammonia excretion depended significantly on feed ration. An increase of feed ration from 0.2 to 0.8% of fish biomass caused an increase of OC and AE from 126.80 mg O₂ kg⁻¹ h⁻¹ and 1.95 mg TAN kg⁻¹ h⁻¹ to 187.35 mg O₂ kg⁻¹ h⁻¹ and 8.80 mg TAN kg⁻¹ h⁻¹ (p<0.05). There was no dependence between feeding frequency and the mean rate of oxygen consumption. However, the relationship between feeding frequency and ammonia excretion by juvenile tench was statistically significant (p<0.05). Feeding frequency significantly affected daily fluctuations of AE and OC. It was found that diurnal variations in metabolic rates were strictly related to tench feeding, and the daily variations of AE were significantly higher than OC.     

Effect of stocking density on survival and growth performance of pikeperch, <Emphasis Type="Italic">Sander lucioperca</Emphasis> (L.), larvae under controlled conditions

The effect of stocking density on the survival and growth of pikeperch, Sander lucioperca (L.), larvae was examined in two consecutive experiments. In experiment I, 4-day-old larvae [body wet weight (BW): 0.5 mg; total body length (TL): 5.6 mm] were reared in 200-l cylindro-conical tanks in a closed, recirculating system (20 ± 0.5°C) at three stocking densities (25, 50 and 100 larvae l⁻¹) and fed a mixed feed (Artemia nauplii and Lansy A2 artificial feed) for 14 consecutive days. At densities of 25 and 100 larvae l⁻¹, growth rate and survival ranged from 2.7 to 1.9 mg day⁻¹ and from 79.2 to 72.3%, and fish biomass gain ranged from 0.6 to 2.0 g l⁻¹, respectively. There were two periods of increased larval mortality: the first was at beginning of exogenous feeding and the second during swim bladder inflation. In experiment II, 18-day-old larvae (BW: 35 mg; TL: 15.6 mm) obtained from experiment I were reared under culture conditions similar to those of experiment I, but at lower stocking densities (6, 10 and 15 larvae l⁻¹). The fish were fed exclusively with artificial feed (trout starter) for 21 consecutive days. At densities of 6 and 15 larvae l⁻¹, the growth rate and fish biomass gain ranged from 28.8 to 23.1 mg day⁻¹ and from 2.0 to 3.3 g l⁻¹, respectively. The highest survival (56.5%) was achieved at a density of 6 larvae l⁻¹. Mortality at all densities was mainly caused by cannibalism II type behaviour (27–35% of total). In both experiments, growth and survival were negatively correlated and fish biomass gain positively correlated with stocking densities. The present study suggests that the initial stocking density of pikeperch larvae reared in a recirculating system can be 100 individuals l⁻¹ for the 4- to 18-day period post-hatch and 15 individuals l⁻¹ for the post-19-day period.     

Holding the line: three decades of prescribed fires halt but do not reverse woody encroachment in grasslands

Context

Encroachment of woody vegetation represents a significant global threat to biodiversity in grasslands, but practices used to reverse encroachment are rarely evaluated comprehensively. Several factors may drive encroachment, such as land use history, alteration of disturbance regimes, and local environment, but their relative importance is poorly understood. Another complicating factor is that encroachment may proceed via positive feedbacks that result in thresholds, beyond which its reversal is difficult.

Objectives

We ask what impact reintroducing frequent fire has on encroachment relative to the influences of landscape context and historical vegetation. We investigate whether woody cover frequency distributions suggest that feedbacks reinforce encroachment after a threshold of woody cover is surpassed.

Methods

We analyze aerial photos in glade grasslands in Missouri, USA, to assess encroachment patterns over a 75-year period. Fire was excluded from this landscape for the first 45 years, and then reintroduced at varying frequencies in the last 30 years.

Results

Woody vegetation cover increased sevenfold from 1939 to 2014 overall. After the reintroduction of prescribed fire, woody cover stayed approximately constant in burned glades, but continued increasing in unburned glades. Woody cover followed bimodal frequency distributions in burned areas. Fire-tolerant vegetation tended to encroach near historically wooded areas, while fire-sensitive vegetation responded more to fire history.

Conclusions

Altered disturbance regimes, in addition to numerous recognized drivers, can cause ecosystem state changes associated with losses to biodiversity. Conducting management early in the encroachment process and restoring grasslands at broad landscape scales may help counteract local feedbacks that promote encroachment.

     

Point mutations in BHV-1 Us3 gene abolish its ability to induce cytoskeletal changes in various cell types

The Us3 gene is conserved among alphaherpesviruses and codes for a protein kinase, a multifunctional protein involved in many phases of virus infection, like nuclear egress, modulation of apoptosis and modification of the cellular cytoskeleton. Bovine herpesvirus (BHV-1), a member of the Alphaherpesvirinae, contains an open reading frame homologous to Us3 of other herpesviruses, which has been identified as a serine/threonine kinase (Takashima, Y., Tamura, H., Xuan, X., Otsuka, H., 1999. Identification of the Us3 gene product of BHV-1 as a protein kinase and characterization of BHV-1 mutants of the Us3 gene. Virus Res. 59, 23–34). To study the activity of BHV-1 Us3, we have cloned its sequence under control of the human cytomegalovirus (HCMV) promoter/enhancer and introduced it into a recombinant baculovirus (Bac Us3). Confocal microscopy analysis showed profound cytoskeletal modifications in various BHV-1-permissive and non-permissive cells transduced with BacUs3. We observed that Us3 expression changed cellular shape and induced formation of long microtubule-containing cell projections, a phenomenon which had also been observed in cells expressing pseudorabies virus Us3. The intracellular localization of Us3 was mostly nuclear but when the protein accumulated it could be detected in the cytoplasm, cell membranes and projections. Mutated forms of BHV-1 Us3 with point mutations near or within the kinase catalytic domain did not affect cell morphology indicating that kinase activity of BHV-1 Us3 is required for its cytoskeleton remodelling function.     

Presence of Caffeic Acid in Flaxseed Lignan Macromolecule

Phenolic compounds were extracted from defatted flaxseeds using ethanol-dioxane (1:1, v/v). The crude extract obtained was purified using Amberlite XAD-16 column chromatography with water and methanol as mobile phases. RP-HPLC and SE-HPLC showed a lignan macromolecule (LM) as a dominant phenolic compound in the purified extract. After the alkaline hydrolysis of LM caffeic acid glucoside (CaAG) was isolated using a semi-preparative HPLC and its structure was confirmed by LC-ESI-MS. In LM of the investigated flaxseed, one molecule of caffeic acid corresponded with five molecules of p-coumaric acid and two molecules of ferulic acid. The presence of caffeic acid in the lignan molecule might be very beneficial due to its high antioxidant activity.     

Impact of diets with different proportions of linseed and sunflower oils on the growth, liver histology, immunological and chemical blood parameters, and proximate composition of pikeperch Sander lucioperca (L.)

The aim of the study was to determine the impact of applying different proportions of linseed (LO) and sunflower (SFO) oils in pikeperch diets on growth, histological changes in the liver, immunological and blood chemical parameters. The fish were fed isoenergetic and isoprotein feeds containing SFO (group 100SFO) or LO (group 100LO) in quantities of 67 g kg/feed, and a mixture of oils: 47 g SFO and 20 g LO kg/feed (group 70SFO/30LO) and 20 g SFO and 47 g LO kg/feed (group 30SFO/70LO). Dietary ratios of polyunsaturated fatty acids from the n-3 and n-6 series (n3/n6 index) were 0.36–2.15. Pikeperch were reared for 56 days in three replicates for each dietary treatment. Various dietary oils and ratios of n3/n6 did not impact fish growth, feed conversion ratio, viscerosomatic and hepatosomatic index, and size of the hepatocytes. Feeding the fish high quantities of LO and SO oils (groups 100LO and 100SFO) reduced the immunological response of the phagocytes and lymphocytes in the fish. Moreover, this resulted in significant differences among groups in the quantity of linolenic and linoleic acid in whole fish bodies, viscera, fillets, and livers. Various quantities of vegetable oils in the fish diets did not impact the quantity of arachidonic, eicosapentaenoic and docosahexaenoic acid in the fillets and livers. The immunological index and low quantities of linoleic acid in the fillets obtained in group 30SFO/70LO indicate that the n3/n6 dietary ratio of 1.35 was the most advantageous for feeding juvenile pikeperch feeds with vegetable oils.